Journal: Journal of Histochemistry and Cytochemistry

Article Title: Analysis of Cell Type-specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag-transgenic Mice

doi: 10.1369/jhc.2009.954628

Figure Lengend Snippet: Expression level of CK1ɛ in different tissues and organs of 4–6-week-old BALB/c mice. Specimens were homogenized, and equal protein amounts (75 μg) were separated by SDS-PAGE. CK1ɛ protein levels were detected by Western blotting using a CK1ɛ-specific monoclonal antibody (BD Biosciences). Equal loading of extracted proteins was determined by probing a second membrane with a β-actin–specific monoclonal antibody.

Article Snippet: Profiling of CK1ɛ expression levels was done as described by the manufacturer (SuperArray Bioscience Corp.).

Techniques: Expressing, SDS Page, Western Blot, Membrane

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Analysis of Cell Type-specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag-transgenic Mice

doi: 10.1369/jhc.2009.954628

Figure Lengend Snippet: CK1ɛ expression in the gastrointestinal tract, endocrine glands, lung, mammary gland, skin, and urogenital tract. Fixative: acid formalin, fixation by immersion. Peroxidase reaction, dye: DAB. CK1ɛ-specific serum 712 was used for immunohistochemical staining of the following organs: esophagus (A), stomach (B1,B2), small intestine (C), colon (D), salivary gland (E), pancreas (F), liver (G), adrenal gland (H), thyroid gland (I), trachea (J), lung (K1,K2), skin (L), mammary gland (M1,M2), kidney (N1,N2,N3), ureter (O), ovary (P1,P2), uterus (Q), vagina (R), testis (S), epididymis (T), and prostate (U). In B2, arrows point to parietal cells. In C and D, arrows point to goblet cells. In E, arrow indicates the CK1ɛ-positive ductal epithelium. In the inset of F, a pancreatic duct is shown. In the liver (G) the arrow points to a central vein. An intrahepatic bile duct is shown in the inset of G. In H, the numbers indicate the medulla (1), the x zone (2), the zona fasciculata (3), and the zona glomerulosa (4). In K1, arrow indicates the bronchial epithelium. In K2, arrows point to type II pneumocytes. Arrows in M1 indicate the ductal epithelium of the mammary gland. N1 shows the renal cortex, N2 a glomerulum, and N3 the renal medulla. Arrows in O point to umbrella cells of the urothelium. In P1, the numbers indicate the moderate CK1ɛ-positive epithelium of a primary follicle (1) and a secondary follicle (2). The small primordial follicles are marked by arrows. P2 shows several ovarian follicles of different maturation stages at high magnification. In Q, smooth-muscle cells of the myometrium (M) and the epithelium of the endometrium (E) are CK1ɛ positive. In S, asterisk indicates a group of Leydig cells, and arrows indicate spermatogonia. Calibration bars in all figures are as indicated.

Article Snippet: Profiling of CK1ɛ expression levels was done as described by the manufacturer (SuperArray Bioscience Corp.).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Analysis of Cell Type-specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag-transgenic Mice

doi: 10.1369/jhc.2009.954628

Figure Lengend Snippet: CK1ɛ expression in the nervous system. Fixative: acid formalin, fixation by immersion. Peroxidase reaction, dye: DAB. CK1ɛ expression in the hypophysis, the peripheral nervous system, and the central nervous system. (A) Cross-section of the hypophysis shows a strong CK1ɛ expression in the adenohypophysis (AH), neurohypophysis (NH), as well as in the intermediate part (IP). (B) Higher magnification of the adenohypophysis exhibits epithelial cells showing a strong cytoplasmic staining with anti-CK1ɛ. (C) Pituicytes in the neurohypophysis (arrows) show a strong coloration of the cytoplasm, including their processes. The neuropil is moderately stained. (D,E) Nerve cells in the ganglion trigeminale (D) and the spinal ganglion (E) are strongly labeled with anti-CK1ɛ in the cytoplasm. Schwann cells are only weakly marked. An axonal staining was not observed. (F) Saggital section of a mouse brain stained with anti-CK1ɛ. Nerve cells in all cortical layers are significantly stained, as well as neurons of the hippocampus (HC), olfactory bulb (OB), basal ganglia (BG), hypothalamus (Hypothal), and thalamus (Thal). In the midbrain (MB), pons, and medulla oblongata, neurons of specific nuclei express CK1ɛ [e.g., substantia nigra (SN), pontine gray (PG), superior olivary complex (SOC), nucleus of the facial nerve (VII), and paragigantocellular nucleus, lateral part (PGRNl)], as well as neurons of the superior (SCOLL) and inferior collicles (ICOLL). (G) Neurons express CK1ɛ in their cytoplasm, as shown, for example, in the parietal cortex. (H,I) Granule cells and Purkinje cells of the cerebellum do not exhibit CK1ɛ in detectable amounts. Here, astrocytes, including Bergmann glial cells, express CK1ɛ in their cytoplasm. Calibration bar in I is valid for A = 150 μm; B,G = 20 μm; C,I = 25 μm; D,E,H = 50 μm; F = 650 μm.

Article Snippet: Profiling of CK1ɛ expression levels was done as described by the manufacturer (SuperArray Bioscience Corp.).

Techniques: Expressing, Staining, Labeling

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Analysis of Cell Type-specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag-transgenic Mice

doi: 10.1369/jhc.2009.954628

Figure Lengend Snippet: CK1ɛ expression in immobile cells of mesenchymal origin, in hematopoetic and lymphoid organs, and in the eye. Fixative: acid formalin, fixation by immersion. Peroxidase reaction, dye: DAB. CK1ɛ-specific antiserum 712 was used for immohistochemical staining of the following organs/tissues: skeletal muscle (A1,A2); myocardium (B); smooth muscle (C); arteriole, vein, and lymphatic vessel (D1); artery (D2); mesothelial cells of the pleura (E); brown (F1) and white (F2) fatty tissue; hyaline cartilage (G); osteoblasts and osteocytes (H,I); bone marrow (J); spleen (K); thymus (L); lymph node with primary (M) and secondary lymphoid follicle (N); cecal patch (O); eye (P); and retina (Q). In B, arrows indicate intercalated discs of cardiomyocytes. In D1, arrow indicates an arteriole, and numbers indicate a lymphatic vessel (1) and a vein (2). Arrows in H mark highly CK1ɛ-positive osteoblasts. In K, arrows outline a periarteriolar lymphoid sheath of the white pulp. Arrows in L delineate the medullary portion of the thymus. Arrows in M indicate strong CKIɛ-positive macrophages in the subcapsular sinus. In O, the B- and T-cell zones are marked with B and T, respectively. In P, arrow indicates the ciliary body. Arrowheads in Q indicate the inner segment of the photoreceptors, arrows point to the outer plexiform layer, and asterisk indicates the inner plexiform layer. Calibration bars in A–Q are as indicated. Immunohistochemical analysis of frozen retinal sections revealed localization of CK1ɛ in retinal ganglion cells (R,T, red), which were specifically costained with an anti-βIII-tubulin antibody (S,T, green) or DAPI (T, blue). ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; GCL, retinal ganglion cell layer. Bar in T = 50 μm for R–T.

Article Snippet: Profiling of CK1ɛ expression levels was done as described by the manufacturer (SuperArray Bioscience Corp.).

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Analysis of Cell Type-specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag-transgenic Mice

doi: 10.1369/jhc.2009.954628

Figure Lengend Snippet: CK1ɛ expression in mammary epithelium is downregulated upon neoplastic transformation (A). For immunoperoxidase staining of CK1ɛ in normal mammary tissue (left), DCIS (middle), and invasive tumors (right) of WAP-T mice, CK1ɛ-specific antiserum 712 was used. Counterstaining with hemalaun was performed in all tissue sections. Immunostaining of T-Ag is shown in the top row, and CK1ɛ staining is shown in the bottom row. High CK1ɛ expression levels were detected in the cytoplasm of normal ductal and acinar epithelia cells (bottom left), whereas medium CK1ɛ levels were detected in ductal carcinoma in situ (bottom middle). The lowest expression was found in poorly differentiated tumors (bottom right). (B) Total RNA isolated from mammary tissue was reverse transcribed into cDNA. Expression of CK1ɛ was determined by real-time PCR. Relative CK1ɛ expression in breast carcinoma of induced WAP-T mice (black column) is compared with normal mammary tissue of induced WAP-T mice (gray column) and to mammary tissue of non-transgenic animals (white column).

Article Snippet: Profiling of CK1ɛ expression levels was done as described by the manufacturer (SuperArray Bioscience Corp.).

Techniques: Expressing, Transformation Assay, Immunoperoxidase Staining, Immunostaining, Staining, In Situ, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Transgenic Assay

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Analysis of Cell Type-specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag-transgenic Mice

doi: 10.1369/jhc.2009.954628

Figure Lengend Snippet: CK1ɛ expression in normal tissue, in situ carcinoma, and invasive tumors of SV40 T-Ag–induced transgenic mice

Article Snippet: Profiling of CK1ɛ expression levels was done as described by the manufacturer (SuperArray Bioscience Corp.).

Techniques: Expressing, In Situ, Transgenic Assay

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Analysis of Cell Type-specific Expression of CK1? in Various Tissues of Young Adult BALB/c Mice and in Mammary Tumors of SV40 T-Ag-transgenic Mice

doi: 10.1369/jhc.2009.954628

Figure Lengend Snippet: Cell type–specific differences in the expression level of CK1δ and -ɛ

Article Snippet: Profiling of CK1ɛ expression levels was done as described by the manufacturer (SuperArray Bioscience Corp.).

Techniques: Expressing